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CD25兔單克隆抗體

CD25兔單克隆抗體

  • 價(jià)格￥1000
  • 規(guī)格100ul
  • 貨號(hào)HKZ150239
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150239CD25兔單克隆抗體100ul支1000
CD25
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50116?Clone# ?BP6120
Predicted?Molecular Wt:
31kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P01589
Background:
CD25,or?interleukin?2?(IL2)?receptor?alpha?(IL2RA), and?beta?(IL2RB) chains, together?with?the?common?gamma?chain?(IL2RG),?constitute the?high-affinity?IL2?receptor.?Homodimeric alpha chains?(CD25)?result?in?low-affinity?receptor, while?homodimeric?beta?(IL2RB) chains?produce a?medium-affinity?receptor.?Normally an?integral-membrane?protein, soluble?CD25?has?been
isolated?and?determined?to?result?from?extracellular?proteolyisis.?CD25?(the?p55?chain?of?the?interleukin-2?receptor)?is?a ????marker of activation for a?number?of cell?types,?including T?cells,?B?cells,?and?macrophages.?It?also?appears?to?be?a?reliable?immunohistochemical?marker for the discrimination of?neoplastic from?normal/reactive?mast cells,?with?potential?as?a
diagnostic tool?in systemic?mastocytosis. CD25?is also expressed?on?hairy?cell?leukemia.
Subcellular?location:
Membrane
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Recombinant fragment corresponding to?Human?IL-2
Receptor?alpha?with?aa1-250?was?used?as?an immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ??N.A. Azab, et?al. Clinical?Immunology.?2008?127(2):151–157.
2.?Zhang?B, et?al. Ann?Rheum?Dis?2008;67:1037-1040.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of
colon carcinoma?tissue?labelling?CD25?with
BP6120.?Heat?mediated antigen?retrieval was
performed?using
Tris/EDTA?buffer?pH?9.0

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