\n
\n![\"DOG1\u5154\u5355\u514b\u9686\u6297\u4f53\"\/](\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\")
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![\"DOG1\u5154\u5355\u514b\u9686\u6297\u4f53\"\/](\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/20241015165734796.png?imageView2\/0\/format\/webp\/q\/75\")
<\/a><\/div><\/div>\nDOG1\u5154\u5355\u514b\u9686\u6297\u4f53<\/h1><\/div>
DOG1\u5154\u5355\u514b\u9686\u6297\u4f53<\/h1><\/div><\/i>\u4e00\u6297<\/a> \u6297\u4f53<\/a> \u8bd5\u5242<\/a> <\/span><\/div><\/header>- \u4ef7\u683c<\/span>\uffe51000<\/span><\/li>
- \u89c4\u683c<\/span>100ul<\/span><\/li>
- \u8d27\u53f7<\/span>HKZ150150<\/span><\/li>
- \u5355\u4f4d<\/span>\u652f<\/span><\/li>
- \u54c1\u724c<\/span>HaoKebio<\/span><\/li><\/ul>\n\n\n\nQQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n
<\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div>
<\/div><\/div><\/section>\n\n\n\n<\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div>\n\n\u54c1\u724c<\/strong><\/th> \u5546\u54c1\u7f16\u7801<\/strong><\/th> \u5546\u54c1\u540d\u79f0<\/strong><\/th> \u89c4\u683c<\/strong><\/th> \u5355\u4f4d<\/strong><\/th> \u5355\u4ef7<\/strong><\/th><\/tr> HaoKebio<\/th> HKZ150150<\/th> DOG1\u5154\u5355\u514b\u9686\u6297\u4f53<\/th> 100ul<\/th> \u652f<\/th> 1000<\/th><\/tr><\/tbody><\/table>DOG1<\/strong>
Recombinant\u00a0Rabbit\u00a0Monoclonal Antibody\u00a0Product\u00a0Datasheet<\/strong>
Catalog# \u00a0<\/strong>BX50027\u00a0Clone# \u00a0<\/strong>BP6032
Predicted\u00a0Molecular Wt:<\/strong>
114kDa
Purity: \u00a0\u00a0<\/strong>ProA\u00a0affinity\u00a0purified\u00a0IgG
Species Cross-reactivity:<\/strong>
Human
Form: \u00a0\u00a0<\/strong>Liquid
Applications:<\/strong>
IHC-P
Swissprot\u00a0ID:\u00a0\u00a0<\/strong>Q5XXA6
Background:<\/strong>
DOG1\u00a0is\u00a0a\u00a0calcium-dependent\u00a0chloride\u00a0channel\u00a0protein\u00a0that\u00a0is\u00a0encoded\u00a0by\u00a0a\u00a0gene\u00a0called\u00a0TMEM16A (TMEM16\u00a0FLJ10261,\u00a0ANO1,\u00a0\u00a0ORAOV2, and\u00a0AOS2)\u00a0located\u00a0on\u00a0chromosome\u00a011q13.\u00a0DOG1\u00a0has\u00a0many\u00a0significant\u00a0functions\u00a0such\u00a0as\u00a0regulation\u00a0of\u00a0the\u00a0cholinergic activity of gastrointestinal smooth\u00a0muscle and\u00a0regulation of\u00a0both\u00a0the\u00a0survival\u00a0and\u00a0proliferation\u00a0of\u00a0cells.
DOG1\u00a0is detected\u00a0in gastrointestinal Cajal cells,\u00a0acinic\u00a0cells\u00a0in\u00a0salivary glands\u00a0(apical\u00a0membraneous\u00a0staining,\u00a0particularly\u00a0in
serous cells),\u00a0pancreatic centroacinar cells,\u00a0liver cells,\u00a0and\u00a0epithelium\u00a0of\u00a0biliary tract,\u00a0breast,\u00a0stomach,\u00a0and\u00a0prostate.\u00a0More\u00a0than\u00a090% of\u00a0all\u00a0gastrointestinal\u00a0stromal\u00a0tumors\u00a0(GISTs)\u00a0are\u00a0DOG1 positive,\u00a0irrespective\u00a0of\u00a0kit\u00a0mutation\u00a0and\u00a0CD117\u00a0positivity. The
staining\u00a0pattern varies from cytoplasmic to\u00a0membranous, with\u00a0usually strong,\u00a0diffuse\u00a0intensity.
DOG1\u00a0is an\u00a0important\u00a0marker\u00a0in the\u00a0identification of GIST together with\u00a0CD117,\u00a0slightly\u00a0more\u00a0sensitive\u00a0(particularly\u00a0in\u00a0gastric
GIST without c-kit\u00a0mutation) and also\u00a0more\u00a0specific than\u00a0CD117.\u00a0DOG1\u00a0is\u00a0also\u00a0useful\u00a0in\u00a0the\u00a0classification\u00a0of\u00a0salivary\u00a0carcinomas,\u00a0and\u00a0pancreatic and\u00a0renal tumors.
Subcellular\u00a0location:<\/strong>
Membrane\/Cytoplasm
Recommended\u00a0method:<\/strong>
Heat\u00a0induced epitope\u00a0retrieval with Tris-EDTA\u00a0buffer\u00a0\u00a0\u00a0(pH\u00a09.0),\u00a0primary\u00a0antibody\u00a0incubate\u00a0at\u00a0RT\u00a0(18\u00b0C-25\u00b0C)\u00a0for\u00a030\u00a0minutes.
Immunogen:<\/strong>
Synthetic\u00a0peptide corresponding to\u00a0DOG1\u00a0residues\u00a0\u00a0\u00a0\u00a0\u00a0within\u00a0aa1-100 of\u00a0DOG1 was\u00a0used\u00a0as\u00a0an\u00a0immunogen.
Storage\u00a0Buffer:<\/strong>
PBS\u00a059%, Sodium\u00a0azide\u00a00.01%,\u00a0Glycerol\u00a040%,\u00a0BSA 0.05%.
Storage conditions:<\/strong>
-25\u00b0C\u00a0to\u00a0-18\u00b0C
Storage instructions:<\/strong>
Shipped on\u00a0blue\u00a0ice.\u00a0Upon delivery,\u00a0aliquot,\u00a0and\u00a0store\u00a0at\u00a0-25\u00b0C\u00a0to\u00a0-18\u00b0C. Avoid\u00a0freeze\u00a0\/\u00a0thaw\u00a0cycles.
Recommended\u00a0Dilutions:<\/strong>
IHC-P:\u00a0\u00a0\u00a0\u00a0\u00a01:100-1:200
Background\u00a0References:<\/strong>
Immunohistochemistry
(Formalin\/PFA-fixed
paraffin-embedded
sections analysis\u00a0of\u00a0GIST\u00a0\u00a0\u00a0\u00a0\u00a0tissue\u00a0labelling\u00a0DOG1 with\u00a0BP6032.\u00a0Heat\u00a0mediated
antigen\u00a0retrieval\u00a0was\u00a0performed\u00a0using
Tris\/EDTA\u00a0buffer\u00a0pH\u00a09.0
1.\u00a0Jung\u00a0SH, et.al, Gut\u00a0Liver.\u00a02011 Jun;5(2):171-80.
2. \u00a0Bergmann\u00a0F, et.al,\u00a0Hum\u00a0Pathol.\u00a02011 Jun;42(6):817-23.<\/figcaption><\/figure>\n\n\n\nHKZ150150_BP6032_DOG1<\/a>\u4e0b\u8f7d<\/a><\/div>\n<\/div>\n\n\n\n\n<\/p>\n\n\n\n
\n\n<\/p>\n<\/div>\n<\/div>\n\n\n\n
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\n\u6682\u65e0<\/p>\n\n\n\n
\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3>- <\/i><\/a>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><\/div><\/section>\n\n\n\n
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- \u4ef7\u683c<\/span>\uffe51000<\/span><\/li>
- \u89c4\u683c<\/span>100ul<\/span><\/li>
- \u8d27\u53f7<\/span>HKZ150150<\/span><\/li>
- \u5355\u4f4d<\/span>\u652f<\/span><\/li>
- \u54c1\u724c<\/span>HaoKebio<\/span><\/li><\/ul>\n\n\n\nQQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n
<\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div>
<\/div><\/div><\/section>\n\n\n\n<\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div>\n\n\u54c1\u724c<\/strong><\/th> \u5546\u54c1\u7f16\u7801<\/strong><\/th> \u5546\u54c1\u540d\u79f0<\/strong><\/th> \u89c4\u683c<\/strong><\/th> \u5355\u4f4d<\/strong><\/th> \u5355\u4ef7<\/strong><\/th><\/tr> HaoKebio<\/th> HKZ150150<\/th> DOG1\u5154\u5355\u514b\u9686\u6297\u4f53<\/th> 100ul<\/th> \u652f<\/th> 1000<\/th><\/tr><\/tbody><\/table> DOG1<\/strong>
Recombinant\u00a0Rabbit\u00a0Monoclonal Antibody\u00a0Product\u00a0Datasheet<\/strong>
Catalog# \u00a0<\/strong>BX50027\u00a0Clone# \u00a0<\/strong>BP6032
Predicted\u00a0Molecular Wt:<\/strong>
114kDa
Purity: \u00a0\u00a0<\/strong>ProA\u00a0affinity\u00a0purified\u00a0IgG
Species Cross-reactivity:<\/strong>
Human
Form: \u00a0\u00a0<\/strong>Liquid
Applications:<\/strong>
IHC-P
Swissprot\u00a0ID:\u00a0\u00a0<\/strong>Q5XXA6
Background:<\/strong>
DOG1\u00a0is\u00a0a\u00a0calcium-dependent\u00a0chloride\u00a0channel\u00a0protein\u00a0that\u00a0is\u00a0encoded\u00a0by\u00a0a\u00a0gene\u00a0called\u00a0TMEM16A (TMEM16\u00a0FLJ10261,\u00a0ANO1,\u00a0\u00a0ORAOV2, and\u00a0AOS2)\u00a0located\u00a0on\u00a0chromosome\u00a011q13.\u00a0DOG1\u00a0has\u00a0many\u00a0significant\u00a0functions\u00a0such\u00a0as\u00a0regulation\u00a0of\u00a0the\u00a0cholinergic activity of gastrointestinal smooth\u00a0muscle and\u00a0regulation of\u00a0both\u00a0the\u00a0survival\u00a0and\u00a0proliferation\u00a0of\u00a0cells.
DOG1\u00a0is detected\u00a0in gastrointestinal Cajal cells,\u00a0acinic\u00a0cells\u00a0in\u00a0salivary glands\u00a0(apical\u00a0membraneous\u00a0staining,\u00a0particularly\u00a0in
serous cells),\u00a0pancreatic centroacinar cells,\u00a0liver cells,\u00a0and\u00a0epithelium\u00a0of\u00a0biliary tract,\u00a0breast,\u00a0stomach,\u00a0and\u00a0prostate.\u00a0More\u00a0than\u00a090% of\u00a0all\u00a0gastrointestinal\u00a0stromal\u00a0tumors\u00a0(GISTs)\u00a0are\u00a0DOG1 positive,\u00a0irrespective\u00a0of\u00a0kit\u00a0mutation\u00a0and\u00a0CD117\u00a0positivity. The
staining\u00a0pattern varies from cytoplasmic to\u00a0membranous, with\u00a0usually strong,\u00a0diffuse\u00a0intensity.
DOG1\u00a0is an\u00a0important\u00a0marker\u00a0in the\u00a0identification of GIST together with\u00a0CD117,\u00a0slightly\u00a0more\u00a0sensitive\u00a0(particularly\u00a0in\u00a0gastric
GIST without c-kit\u00a0mutation) and also\u00a0more\u00a0specific than\u00a0CD117.\u00a0DOG1\u00a0is\u00a0also\u00a0useful\u00a0in\u00a0the\u00a0classification\u00a0of\u00a0salivary\u00a0carcinomas,\u00a0and\u00a0pancreatic and\u00a0renal tumors.
Subcellular\u00a0location:<\/strong>
Membrane\/Cytoplasm
Recommended\u00a0method:<\/strong>
Heat\u00a0induced epitope\u00a0retrieval with Tris-EDTA\u00a0buffer\u00a0\u00a0\u00a0(pH\u00a09.0),\u00a0primary\u00a0antibody\u00a0incubate\u00a0at\u00a0RT\u00a0(18\u00b0C-25\u00b0C)\u00a0for\u00a030\u00a0minutes.
Immunogen:<\/strong>
Synthetic\u00a0peptide corresponding to\u00a0DOG1\u00a0residues\u00a0\u00a0\u00a0\u00a0\u00a0within\u00a0aa1-100 of\u00a0DOG1 was\u00a0used\u00a0as\u00a0an\u00a0immunogen.
Storage\u00a0Buffer:<\/strong>
PBS\u00a059%, Sodium\u00a0azide\u00a00.01%,\u00a0Glycerol\u00a040%,\u00a0BSA 0.05%.
Storage conditions:<\/strong>
-25\u00b0C\u00a0to\u00a0-18\u00b0C
Storage instructions:<\/strong>
Shipped on\u00a0blue\u00a0ice.\u00a0Upon delivery,\u00a0aliquot,\u00a0and\u00a0store\u00a0at\u00a0-25\u00b0C\u00a0to\u00a0-18\u00b0C. Avoid\u00a0freeze\u00a0\/\u00a0thaw\u00a0cycles.
Recommended\u00a0Dilutions:<\/strong>
IHC-P:\u00a0\u00a0\u00a0\u00a0\u00a01:100-1:200
Background\u00a0References:<\/strong>
Immunohistochemistry
(Formalin\/PFA-fixed
paraffin-embedded
sections analysis\u00a0of\u00a0GIST\u00a0\u00a0\u00a0\u00a0\u00a0tissue\u00a0labelling\u00a0DOG1 with\u00a0BP6032.\u00a0Heat\u00a0mediated
antigen\u00a0retrieval\u00a0was\u00a0performed\u00a0using
Tris\/EDTA\u00a0buffer\u00a0pH\u00a09.0
1.\u00a0Jung\u00a0SH, et.al, Gut\u00a0Liver.\u00a02011 Jun;5(2):171-80.
2. \u00a0Bergmann\u00a0F, et.al,\u00a0Hum\u00a0Pathol.\u00a02011 Jun;42(6):817-23.<\/figcaption><\/figure>\n\n\n\nHKZ150150_BP6032_DOG1<\/a>\u4e0b\u8f7d<\/a><\/div>\n<\/div>\n\n\n\n\n<\/p>\n\n\n\n
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