{"id":4532,"date":"2023-07-14T11:19:37","date_gmt":"2023-07-14T03:19:37","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=4532"},"modified":"2024-09-26T16:42:25","modified_gmt":"2024-09-26T08:42:25","slug":"e-cadherin%e5%85%94%e5%8d%95%e5%85%8b%e9%9a%86%e6%8a%97%e4%bd%93-2","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/4532.html","title":{"rendered":"E-Cadherin\u5154\u5355\u514b\u9686\u6297\u4f53"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"E-Cadherin\u5154\u5355\u514b\u9686\u6297\u4f53\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">E-Cadherin\u5154\u5355\u514b\u9686\u6297\u4f53<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/kt\/yk\/\">\u4e00\u6297<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/kt\/\">\u6297\u4f53<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\uffe51125<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">100ul<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKI0391A<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u652f<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<figure class=\"wp-block-table\"><table><tbody><tr><th><strong>\u54c1\u724c<\/strong><\/th><th><strong>\u5546\u54c1\u7f16\u7801<\/strong><\/th><th><strong>\u5546\u54c1\u540d\u79f0<\/strong><\/th><th><strong>\u89c4\u683c<\/strong><\/th><th><strong>\u5355\u4f4d<\/strong><\/th><th><strong>\u5355\u4ef7<\/strong><\/th><\/tr><tr><th>HaoKebio<\/th><th>HKI0391A<\/th><th>E-Cadherin\u5154\u5355\u514b\u9686\u6297\u4f53<\/th><th>100ul<\/th><th>\u652f<\/th><th>1125<\/th><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\">Applications<\/h3>\n\n\n\n<p><strong>Tested Applications:<\/strong><\/p>\n\n\n\n<p>FC, IF, IHC, WB,ELISA<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Cited Applications:<\/strong><\/p>\n\n\n\n<p>FC, IF, IHC, WB<\/p>\n\n\n\n<p><strong>Species Specificity:<\/strong><\/p>\n\n\n\n<p>human, pig, rat<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Cited Species:<\/strong><\/p>\n\n\n\n<p>human, monkey, mouse, pig<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Positive WB detected in:<\/strong><\/p>\n\n\n\n<p>PC-3 cells, A431 cells, MCF-7 cells, pig brain tissue, MKN-45 cells, SGC-7901 cells<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Positive IHC detected in:<\/strong><\/p>\n\n\n\n<p>human breast cancer tissue, human colon tissue, rat colon tissue<\/p>\n\n\n\n<p><strong><em>Note: suggested antigen retrieval with&nbsp;TE buffer pH 9.0;&nbsp;(*) Alternatively, antigen retrieval may be performed with&nbsp;citrate buffer pH 6.0<\/em><\/strong><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Positive IF detected in:<\/strong><\/p>\n\n\n\n<p>human breast cancer tissue, human kidney tissue<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Positive FC detected in:<\/strong><\/p>\n\n\n\n<p>A431 cells<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Recommended dilution:<\/strong><\/p>\n\n\n\n<p>WB : 1:2000-1:8000<\/p>\n\n\n\n<p>IHC : 1:1000-1:4000<\/p>\n\n\n\n<p>IF : 1:200-1:800<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Product Information<\/h3>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Source:<\/strong><\/p>\n\n\n\n<p>Mouse<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Purification method:<\/strong><\/p>\n\n\n\n<p>Protein A purification<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Isotype:<\/strong><\/p>\n\n\n\n<p>IgG2b<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Storage:<\/strong><\/p>\n\n\n\n<p>PBS with 0.02% sodium azide and 50% glycerol pH 7.3.<br>Store at -20\u00b0C. Stable for one year after shipment.&nbsp;Aliquoting is unnecessary for -20<sup>o<\/sup>C storage.<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Full name:<\/strong><\/p>\n\n\n\n<p>cadherin 1, type 1, E-cadherin (epithelial)<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Calculated molecular weight:<\/strong><\/p>\n\n\n\n<p>882 aa, 97 kDa<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Observed molecular weight:<\/strong><\/p>\n\n\n\n<p>120 kDa<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Gene symbol<\/strong><\/p>\n\n\n\n<p>CDH1<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>Synonyms<\/strong><\/p>\n\n\n\n<p>Arc 1, Cadherin 1, CAM 120\/80, CD324, CDH1, CDHE, E cadherin, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVO, Uvomorulin<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Background<\/h3>\n\n\n\n<p>Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120\/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, \u03b1-, \u03b2-, and \u03b3-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Protocols<\/h3>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div class=\"tabbottomb\"><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":7870,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[101,99,98],"tags":[],"class_list":["post-4532","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-yk","category-kt","category-products"],"metadata":{"_wp_page_template":["default"],"_wpcom_metas":["a:1:{s:11:\"show_indent\";s:1:\"0\";}"],"views":["1130"],"_canshu":["a:5:{i:0;a:5:{s:5:\"title\";s:6:\"\u4ef7\u683c\";s:4:\"text\";s:7:\"\uffe51125\";s:2:\"f1\";i:16;s:2:\"f2\";s:7:\"#e21f2f\";s:2:\"f3\";b:1;}i:1;a:5:{s:5:\"title\";s:6:\"\u89c4\u683c\";s:4:\"text\";s:5:\"100ul\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:2;a:5:{s:5:\"title\";s:6:\"\u8d27\u53f7\";s:4:\"text\";s:8:\"HKI0391A\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:3;a:5:{s:5:\"title\";s:6:\"\u5355\u4f4d\";s:4:\"text\";s:3:\"\u652f\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";b:0;}i:4;a:5:{s:5:\"title\";s:6:\"\u54c1\u724c\";s:4:\"text\";s:8:\"HaoKebio\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}}"],"catce":["sidebar-widgets4"],"_dp_original":["4529"],"_edit_lock":["1728531419:2"],"_edit_last":["2"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/4532"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=4532"}],"version-history":[{"count":5,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/4532\/revisions"}],"predecessor-version":[{"id":9557,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/4532\/revisions\/9557"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=4532"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=4532"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=4532"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}