{"id":4099,"date":"2023-07-11T11:41:47","date_gmt":"2023-07-11T03:41:47","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=4099"},"modified":"2024-09-14T17:15:23","modified_gmt":"2024-09-14T09:15:23","slug":"ripa-%e6%80%bb%e8%9b%8b%e7%99%bd%e8%a3%82%e8%a7%a3%e6%b6%b2","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/4099.html","title":{"rendered":"RIPA \u603b\u86cb\u767d\u88c2\u89e3\u6db2"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"padding-bottom:14px;padding-top:28px;\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/hkw2011.png?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/hkw2011.png?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"RIPA \u603b\u86cb\u767d\u88c2\u89e3\u6db2\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">RIPA \u603b\u86cb\u767d\u88c2\u89e3\u6db2<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/wb\/\">\u86cb\u767d\u8d28\u751f\u7269\u5b66<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\u00a5120<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">100ml<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKW2011<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u74f6<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><div class=\"PcOnly themepark-block_layout_ba\"><video id=\"VideoPlayer\" src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" autoplay loop muted defaultmuted=\"\" playsinline><source src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" type=\"video\/mp4\"\/><\/video><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div class=\"tabbottomb\"><\/div><\/div>\n\n\n\n<p style=\"font-size:15px\"><strong>\u3010\u4ea7\u54c1\u7b80\u4ecb\u3011<\/strong><strong><\/strong><\/p>\n\n\n\n<p style=\"font-size:15px\">RIPA \u88c2\u89e3\u6db2\uff08RIPA Lysis Buffer\uff09\u662f\u4e00\u79cd\u4f20\u7edf\u7684\u7ec6\u80de\u53ca\u7ec4\u7ec7\u5feb\u901f\u88c2\u89e3\u6db2\u3002RIPA\u89e3\u6db2\u88c2\u89e3\u7ec4\u7ec7\u3001\u7ec6\u80de\u5f97\u5230\u7684\u86cb\u767d\u6837\u54c1\u53ef\u4ee5\u7528\u4e8e\u5e38\u89c4\u7684 PAGE\u3001 Western \u7b49\u5bf9\u86cb\u767d\u6d3b\u6027\u6ca1\u6709\u4e25\u683c\u8981\u6c42\u7684\u5b9e\u9a8c\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\">\u672c\u4ea7\u54c1\u4e3b\u8981\u6210\u5206\u5305\u542b 50 mM Tris-HCl (pH 7.4)\uff0c150 mM NaCl\uff0c 1 mM EDTA\uff0c1mM EGTA,1% NP-40\u30015%\u7518\u6cb9\u3002\u672c\u4ea7\u54c1\u9002\u7528\u4e8e\u52a8\u7269\u6216\u690d\u7269\u7ec4\u7ec7\u53ca\u7ec6\u80de\u6837\u54c1\uff0c \u4e5f\u53ef\u7528\u4e8e\u771f\u83cc\u6216\u7ec6\u83cc\u6837\u54c1\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\"><strong>\u3010\u4ea7\u54c1\u7ec4\u6210\u00a0\u3011<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\" style=\"font-size:15px\"><table class=\"has-fixed-layout\"><tbody><tr><td>\u4ea7\u54c1\u76ee\u5f55<\/td><td>\u4e3b\u8981\u6210\u5206<\/td><td>\u4ea7\u54c1\u89c4\u683c<\/td><\/tr><tr><td>RIPA \u603b\u86cb\u767d\u88c2\u89e3\u6db2<\/td><td>NP-40<\/td><td>100mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p style=\"font-size:15px\"><strong>\u3010<\/strong><strong>\u50a8\u5b58\u4e0e\u8fd0\u8f93<\/strong><strong>\u3011<\/strong><strong><\/strong><\/p>\n\n\n\n<p style=\"font-size:15px\">\u51b0\u888b\uff08wet ice\uff09\u8fd0\u8f93\uff1b4\u2103\u907f\u5149\u4fdd\u5b58\uff0c\u6709\u6548\u671f 18 \u4e2a\u6708\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\"><strong>\u3010\u4f7f\u7528\u65b9\u6cd5\u3011<\/strong><strong><\/strong><\/p>\n\n\n\n<p style=\"font-size:15px\">\u81ea\u5907\u86cb\u767d\u9176\u6291\u5236\u5242\u3002RIPA \u88c2\u89e3\u6db2\u5728\u4e34\u7528\u524d\u9700\u52a0\u5165\u86cb\u767d\u9176\u6291\u5236\u5242\u9632\u6b62\u86cb\u767d\u964d\u89e3\u3002\u4ee5\u4e0b\u4f7f\u7528\u65b9\u6cd5\u4e2d\u63d0\u5230\u7684 RIPA \u88c2\u89e3\u6db2\u5747\u6307\u5df2\u6dfb\u52a0\u86cb\u767d\u9176\u6291\u5236\u5242\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\"><strong>\u7ec4\u7ec7\u6837\u54c1<\/strong><strong>\u5b9e\u9a8c<\/strong>\uff1a<\/p>\n\n\n\n<p style=\"font-size:15px\">1.\u7ec4\u7ec7\u5757\u7528\u9884\u51b7 PBS\u6d17\u6da4\uff0c\u53bb\u9664\u8840\u6c61\uff0c\u526a\u6210\u7ec6\u5c0f\u788e\u5757\u7f6e\u4e8e\u5300\u6d46\u5668\u4e2d\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\">2. \u52a0\u5165 10 \u500d\u7ec4\u7ec7\u4f53\u79ef RIPA \u88c2\u89e3\u6db2\u4f4e\u6e29\u5300\u6d46\u3002\u6ce8\u610f\uff0cRIPA \u88c2\u89e3\u6db2\u7684\u4f7f\u7528\u91cf\u53ef\u6309\u7167\u7ea6\u6bcf 50 mg \u7ec4\u7ec7\u4e0e 1 mL \u88c2\u89e3\u6db2\u7684\u6bd4\u4f8b\u6dfb\u52a0\u3002\u5982\u7ec4\u7ec7\u86cb\u767d\u542b\u91cf\u8f83\u4f4e\uff0c\u53ef\u964d\u4f4e\u88c2\u89e3\u6db2\u7684\u7528\u91cf\uff0c\u4ee5\u63d0\u9ad8\u7c97\u63d0\u6eb6\u6db2\u4e2d\u7684\u86cb\u767d\u6d53\u5ea6\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\">3.\u5c06\u5300\u6d46\u6db2\u8f6c\u79fb\u81f3 1.5 mL \u79bb\u5fc3\u7ba1\u4e2d\uff0c\u632f\u8361\u3002\u51b0\u6d74 30 min\uff0c\u671f\u95f4\u6bcf 10 min \u7528\u79fb\u6db2\u5668\u53cd\u590d\u5439\u6253\uff0c\u786e\u4fdd\u7ec4\u7ec7\u7ec6\u80de\u5b8c\u5168\u88c2\u89e3\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\">4. 12000&nbsp;g\u79bb\u5fc3 5 min\uff0c\u6536\u96c6\u4e0a\u6e05\uff0c\u5373\u4e3a\u603b\u86cb\u767d\u6eb6\u6db2\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\"><strong>\u8d34\u58c1\u7ec6\u80de\u6837\u54c1<\/strong><strong>\u5b9e\u9a8c<\/strong>\uff1a<\/p>\n\n\n\n<ol style=\"font-size:15px\" class=\"wp-block-list\">\n<li>\u7528 PBS \u6e05\u6d17\u7ec6\u80de 2-3 \u6b21\uff0c\u6700\u540e\u4e00\u6b21\u5f7b\u5e95\u5438\u5e72\u6b8b\u7559\u6db2\u3002<\/li>\n\n\n\n<li>\u6309\u7167 6 \u5b54\u677f\u6bcf\u5b54\u7ec6\u80de 250 \u03bcL \u88c2\u89e3\u6db2\u7684\u6bd4\u4f8b\u5438\u53d6 RIPA \u88c2\u89e3\u6db2\u4e8e\u7ec6\u80de\u57f9\u517b\u677f\u3001\u74f6\u5185\uff0c\u53cd\u590d\u6643\u52a8\u57f9\u517b\u677f\u6216\u74f6\uff0c\u4f7f\u88c2\u89e3\u6db2\u4e0e\u7ec6\u80de\u5145\u5206\u63a5\u89e6 3-5 min\u3002<\/li>\n\n\n\n<li>\u7528\u7ec6\u80de\u522e\u5200\u5c06\u7ec6\u80de\u522e\u4e0b\uff0c\u6536\u96c6\u5230\u79bb\u5fc3\u7ba1\u4e2d\u3002<\/li>\n\n\n\n<li>12000 g \u79bb\u5fc3 5 min\uff0c\u6536\u96c6\u4e0a\u6e05\uff0c\u5373\u4e3a\u603b\u86cb\u767d\u6eb6\u6db2\u3002<\/li>\n<\/ol>\n\n\n\n<p style=\"font-size:15px\"><strong>\u60ac\u6d6e\u7ec6\u80de\u6837\u54c1<\/strong><strong>\u5b9e\u9a8c<\/strong>\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li style=\"font-size:15px\">\u79bb\u5fc3\u6536\u96c6\u7ec6\u80de\u3002<\/li>\n\n\n\n<li style=\"font-size:15px\">\u6309\u7167 6 \u5b54\u677f\u6bcf\u5b54\u7ec6\u80de 250 \u03bcL \u88c2\u89e3\u6db2\u7684\u6bd4\u4f8b\u5c06\u7ec6\u80de\u6db2\u4e0e RIPA \u88c2\u89e3\u6db2\u6df7\u5408\uff0c\u632f\u8361\u3002<\/li>\n\n\n\n<li style=\"font-size:15px\">\u51b0\u6d74 30 min\uff0c\u671f\u95f4\u6bcf 10 min \u7528\u79fb\u6db2\u5668\u53cd\u590d\u5439\u6253\u6570\u6b21\uff0c\u786e\u4fdd\u7ec6\u80de\u5b8c\u5168\u88c2\u89e3\u3002<\/li>\n\n\n\n<li style=\"font-size:15px\">12000 g \u79bb\u5fc3 5 min\uff0c\u6536\u96c6\u4e0a\u6e05\uff0c\u5373\u4e3a\u603b\u86cb\u767d\u6eb6\u6db2\u3002<\/li>\n<\/ol>\n\n\n\n<p style=\"font-size:15px\"><strong>\u7ec6\u83cc\u6216\u771f\u83cc\u6837\u672c<\/strong><strong>\u5b9e\u9a8c<\/strong>\uff1a<\/p>\n\n\n\n<ol style=\"font-size:15px\" class=\"wp-block-list\">\n<li>\u53d6 1 mL \u83cc\u60ac\u6db2\uff0c\u79bb\u5fc3\u53bb\u4e0a\u6e05\uff0cPBS \u6d17\u6da4\u4e00\u6b21\uff0c\u5145\u5206\u53bb\u9664\u6db2\u4f53\u3002\u6da1\u65cb\u4f7f\u83cc\u4f53\u5c3d\u91cf\u5206\u6563\u3002<\/li>\n\n\n\n<li>\u52a0\u5165 100-200 \u03bcL RIPA \u88c2\u89e3\u6db2\uff0c\u8f7b\u8f7b\u6da1\u65cb\u4f7f\u83cc\u4f53\u4e0e\u88c2\u89e3\u6db2\u5145\u5206\u6df7\u5300\u3002<\/li>\n\n\n\n<li>\u51b0\u6d74 10 min\uff0c\u671f\u95f4\u6bcf 2 min \u7528\u79fb\u6db2\u5668\u53cd\u590d\u5439\u6253\u6570\u6b21\uff0c\u786e\u4fdd\u83cc\u4f53\u5b8c\u5168\u88c2\u89e3\u3002<\/li>\n\n\n\n<li>12000 g \u79bb\u5fc3 5 min\uff0c\u6536\u96c6\u4e0a\u6e05\uff0c\u5373\u4e3a\u603b\u86cb\u767d\u6eb6\u6db2\u3002<\/li>\n<\/ol>\n\n\n\n<p style=\"font-size:15px\"><strong>\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/strong><strong><\/strong><\/p>\n\n\n\n<ol style=\"font-size:15px\" class=\"wp-block-list\">\n<li>\u7ec4\u7ec7\u6216\u7ec6\u80de\u88c2\u89e3\u65f6\u82e5\u4f1a\u51fa\u73b0\u7c98\u7a20\u72b6\u3002\u53ef\u7528\u79fb\u6db2\u5668\u53cd\u590d\u5439\u6253\u6216\u632f\u8361\u5668\u632f\u8361\uff0c\u76f4\u81f3\u5448\u6db2\u72b6\u4e3a\u6b62\u3002\u5982\u679c\u4e00\u76f4\u8f83\u7a20\uff0c\u53ef\u518d\u52a0\u5165\u9002\u91cf\u88c2\u89e3\u6db2\u3002<\/li>\n\n\n\n<li>\u672c\u8bd5\u5242\u4e0d\u542b\u6709\u86cb\u767d\u9176\u6291\u5236\u5242\uff0c\u9700\u81ea\u5907\u86cb\u767d\u9176\u6291\u5236\u5242\u5e76\u5728\u4e34\u7528\u524d\u52a0\u5165\u3002<\/li>\n\n\n\n<li>\u64cd\u4f5c\u65f6\u8bf7\u7a7f\u5b9e\u9a8c\u670d\uff0c\u5e76\u4f69\u6234\u4e00\u6b21\u6027\u624b\u5957\u3002<\/li>\n<\/ol>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-2605bc36-4647-4282-91b6-20bcc632ca20\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20240914171432531.pdf\">RIPA \u603b\u86cb\u767d\u88c2\u89e3\u6db2 HKW2011\u8bf4\u660e\u4e66 <\/a><a href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20240914171432531.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-2605bc36-4647-4282-91b6-20bcc632ca20\">\u4e0b\u8f7d<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u3010\u4ea7\u54c1\u7b80\u4ecb\u3011 RIPA \u88c2\u89e3\u6db2\uff08RIPA Lysis Buffer\uff09\u662f\u4e00\u79cd\u4f20\u7edf\u7684\u7ec6\u80de\u53ca\u7ec4\u7ec7\u5feb\u901f\u88c2\u89e3\u6db2\u3002RIPA 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