![\"Cyclin](\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\")
<\/a><\/div><\/div>Cyclin D1 \u5154\u5355\u514b\u9686\u5373\u7528\u578b\u6297\u4f53<\/h1><\/div><\/i>\u4e00\u6297<\/a> \u6297\u4f53<\/a> \u8bd5\u5242<\/a> <\/span><\/div><\/header>- \u4ef7\u683c<\/span>\u00a5270\/\u00a5450<\/span><\/li>
- \u89c4\u683c<\/span>3ml\/6ml<\/span><\/li>
- \u8d27\u53f7<\/span>HKA50044G<\/span><\/li>
- \u5355\u4f4d<\/span>\u652f<\/span><\/li>
- \u54c1\u724c<\/span>HaoKebio<\/span><\/li><\/ul>\n\n\n\nQQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n
<\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div>
<\/div><\/div><\/section>\n\n\n\n<\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div>\n\n\u54c1\u724c<\/strong><\/th> \u5546\u54c1\u7f16\u7801<\/strong><\/th> \u5546\u54c1\u540d\u79f0<\/strong><\/th> \u89c4\u683c<\/strong><\/th> \u5355\u4f4d<\/strong><\/th> \u5355\u4ef7<\/strong><\/th><\/tr> HaoKebio<\/th> HKA50044G<\/th> Cyclin D1 \u5154\u5355\u514b\u9686\u5373\u7528\u578b\u6297\u4f53<\/th> 3ml<\/th> \u652f<\/th> 270<\/th><\/tr> HaoKebio<\/strong><\/td> HKA50044G<\/strong><\/td> Cyclin D1 \u5154\u5355\u514b\u9686\u5373\u7528\u578b\u6297\u4f53<\/strong><\/td> 6ml<\/strong><\/td> \u652f<\/strong><\/td> 450<\/strong><\/td><\/tr><\/tbody><\/table>\u80cc\u666f:<\/strong>
\u7ec6\u80de\u5468\u671f\u86cb\u767d\u4f9d\u8d56\u6027\u6fc0\u9176CDK4\u548cCDK6\u7684\u6d3b\u6027\u53d7\u5230T\u73af\u78f7\u9178\u5316\u3001\u5176\u7ec6\u80de\u5468\u671f\u86cb\u767d\u4f19\u4f34\uff08D\u578b\u7ec6\u80de\u5468\u671f\u86cb\u767d\uff09\u7684\u4e30\u5ea6\u4ee5\u53ca\u4e0eCip\/Kip\u6216INK\u5bb6\u65cf\u86cb\u767d\u7684CDK\u6291\u5236\u5242\u7684\u7ed3\u5408\u7684\u8c03\u63a7\u3002
\u4e0d\u6d3b\u8dc3\u7684\u7ec6\u80de\u5468\u671f\u86cb\u767dD\/CDK4\u548cp27 Kip1\u7684\u4e09\u5143\u590d\u5408\u7269\u9700\u8981\u7ec6\u80de\u5916\u7684\u4fc3\u5206\u88c2\u523a\u6fc0\uff0c\u4ee5\u91ca\u653e\u548c\u964d\u89e3p27\uff0c\u540c\u65f6\u4f34\u968f\u7ec6\u80de\u5468\u671f\u86cb\u767dD\u6c34\u5e73\u7684\u4e0a\u5347\uff0c\u4ece\u800c\u5f71\u54cd\u901a\u8fc7\u9650\u5236\u70b9\u7684\u8fdb\u7a0b\u548cRb\u4f9d\u8d56\u7684S\u671f\u8fdb\u5165\u3002
\u6d3b\u8dc3\u7684\u7ec6\u80de\u5468\u671f\u86cb\u767dD\/CDK4\u590d\u5408\u7269\u9776\u5411\u89c6\u7f51\u819c\u6bcd\u7ec6\u80de\u7624\u86cb\u767d\u8fdb\u884c\u78f7\u9178\u5316\uff0c\u5141\u8bb8E2F\u8f6c\u5f55\u56e0\u5b50\u7684\u91ca\u653e\uff0c\u4ece\u800c\u6fc0\u6d3bG1\/S\u671f\u57fa\u56e0\u8868\u8fbe\u3002\u5728\u751f\u957f\u56e0\u5b50\u64a4\u56de\u540e\uff0c\u7ec6\u80de\u5468\u671f\u86cb\u767dD\u7684\u86cb\u767d\u6c34\u5e73\u901a\u8fc7\u4e0b\u8c03\u86cb\u767d\u8868\u8fbe\u548c\u78f7\u9178\u5316\u4f9d\u8d56\u6027\u964d\u89e3\u800c\u4e0b\u964d\u3002<\/span><\/p>\u4e9a\u7ec6\u80de\u5b9a\u4f4d:<\/strong>
\u7ec6\u80de\u6838\u3002
<\/span>\u63a8\u8350\u65b9\u6cd5:<\/strong>
<\/span>\u9009\u7528Tris-EDTA\u7f13\u51b2\u6db2\uff08\u00a0PH9.0\uff09\u8fdb\u884c\u6297\u539f\u4fee\u590d\uff1b\u00a0\u4e00\u6297\u5728\u5ba4\u6e29\u4e0b\uff084\u2103) \u5b75\u80b2\u8fc7\u591c\u6216\uff0837\u2103\uff09\u6e7f\u76d2\u5b75\u80b22h\u3002
<\/span>\u514d\u75ab\u539f:<\/strong>
<\/span>\u4eba\u7ec6\u80de\u5468\u671f\u86cb\u767dD1\u86cb\u767dAla284\u5468\u56f4\u6b8b\u57fa\u7684\u5408\u6210\u80bd\u3002
<\/span>\u5b58\u50a8\u6eb6\u6db2:<\/strong>
<\/span>10mM HEPES\u94a0(pH 7.5)\u3001150 mM NaCl\u3001100\u00b5g\/ml BSA\u300150%\u7518\u6cb9\u548c\u5c11\u4e8e0.02%\u7684\u53e0\u6c2e\u5316\u94a0\u3002
<\/span>\u50a8\u5b58\u6761\u4ef6:<\/strong>
<\/span>-20\u00b0C\u00a0\u50a8\u5b58\u4e00\u5e74\u3002
<\/span><\/p><\/figcaption><\/figure>\n\n\n\n
\u5b58\u50a8\u8bf4\u660e<\/strong>:<\/strong><\/p>\n\n\n\n
\u4ee5\u84dd\u51b0\u8fd0\u8f93\u3002\u6536\u8d27\u540e\uff0c\u8fdb\u884c\u5206\u88c5\uff0c\u5e76\u5728-25\u00b0C \u81f3-18\u00b0C \u6761\u4ef6\u4e0b\u50a8 \u5b58\u3002\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002<\/p>\n\n\n\n
\u80cc\u666f\u53c2\u8003\u6587\u732e<\/strong>:<\/strong><\/p>\n\n\n\n
\u00a01.Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.<\/p>\n\n\n\n
\u00a02.Sherr, C.J. (1996) Science 274, 1672-7.<\/p>\n\n\n\n
\u00a0<\/p>\n<\/div>\n\n\n\n
\n<\/p>\n\n\n\n
\n\n<\/p>\n<\/div>\n<\/div>\n\n\n\n
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\n\u6682\u65e0<\/p>\n\n\n\n
\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3>- <\/i><\/a>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><\/div><\/section>\n\n\n\n
<\/p>\n<\/div>\n<\/div><\/div>
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- \u4ef7\u683c<\/span>\u00a5270\/\u00a5450<\/span><\/li>
- \u89c4\u683c<\/span>3ml\/6ml<\/span><\/li>
- \u8d27\u53f7<\/span>HKA50044G<\/span><\/li>
- \u5355\u4f4d<\/span>\u652f<\/span><\/li>
- \u54c1\u724c<\/span>HaoKebio<\/span><\/li><\/ul>\n\n\n\nQQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n
<\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div>
<\/div><\/div><\/section>\n\n\n\n<\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div>\n\n\u54c1\u724c<\/strong><\/th> \u5546\u54c1\u7f16\u7801<\/strong><\/th> \u5546\u54c1\u540d\u79f0<\/strong><\/th> \u89c4\u683c<\/strong><\/th> \u5355\u4f4d<\/strong><\/th> \u5355\u4ef7<\/strong><\/th><\/tr> HaoKebio<\/th> HKA50044G<\/th> Cyclin D1 \u5154\u5355\u514b\u9686\u5373\u7528\u578b\u6297\u4f53<\/th> 3ml<\/th> \u652f<\/th> 270<\/th><\/tr> HaoKebio<\/strong><\/td> HKA50044G<\/strong><\/td> Cyclin D1 \u5154\u5355\u514b\u9686\u5373\u7528\u578b\u6297\u4f53<\/strong><\/td> 6ml<\/strong><\/td> \u652f<\/strong><\/td> 450<\/strong><\/td><\/tr><\/tbody><\/table> \u80cc\u666f:<\/strong>
\u7ec6\u80de\u5468\u671f\u86cb\u767d\u4f9d\u8d56\u6027\u6fc0\u9176CDK4\u548cCDK6\u7684\u6d3b\u6027\u53d7\u5230T\u73af\u78f7\u9178\u5316\u3001\u5176\u7ec6\u80de\u5468\u671f\u86cb\u767d\u4f19\u4f34\uff08D\u578b\u7ec6\u80de\u5468\u671f\u86cb\u767d\uff09\u7684\u4e30\u5ea6\u4ee5\u53ca\u4e0eCip\/Kip\u6216INK\u5bb6\u65cf\u86cb\u767d\u7684CDK\u6291\u5236\u5242\u7684\u7ed3\u5408\u7684\u8c03\u63a7\u3002
\u4e0d\u6d3b\u8dc3\u7684\u7ec6\u80de\u5468\u671f\u86cb\u767dD\/CDK4\u548cp27 Kip1\u7684\u4e09\u5143\u590d\u5408\u7269\u9700\u8981\u7ec6\u80de\u5916\u7684\u4fc3\u5206\u88c2\u523a\u6fc0\uff0c\u4ee5\u91ca\u653e\u548c\u964d\u89e3p27\uff0c\u540c\u65f6\u4f34\u968f\u7ec6\u80de\u5468\u671f\u86cb\u767dD\u6c34\u5e73\u7684\u4e0a\u5347\uff0c\u4ece\u800c\u5f71\u54cd\u901a\u8fc7\u9650\u5236\u70b9\u7684\u8fdb\u7a0b\u548cRb\u4f9d\u8d56\u7684S\u671f\u8fdb\u5165\u3002
\u6d3b\u8dc3\u7684\u7ec6\u80de\u5468\u671f\u86cb\u767dD\/CDK4\u590d\u5408\u7269\u9776\u5411\u89c6\u7f51\u819c\u6bcd\u7ec6\u80de\u7624\u86cb\u767d\u8fdb\u884c\u78f7\u9178\u5316\uff0c\u5141\u8bb8E2F\u8f6c\u5f55\u56e0\u5b50\u7684\u91ca\u653e\uff0c\u4ece\u800c\u6fc0\u6d3bG1\/S\u671f\u57fa\u56e0\u8868\u8fbe\u3002\u5728\u751f\u957f\u56e0\u5b50\u64a4\u56de\u540e\uff0c\u7ec6\u80de\u5468\u671f\u86cb\u767dD\u7684\u86cb\u767d\u6c34\u5e73\u901a\u8fc7\u4e0b\u8c03\u86cb\u767d\u8868\u8fbe\u548c\u78f7\u9178\u5316\u4f9d\u8d56\u6027\u964d\u89e3\u800c\u4e0b\u964d\u3002<\/span><\/p>\u4e9a\u7ec6\u80de\u5b9a\u4f4d:<\/strong>
\u7ec6\u80de\u6838\u3002
<\/span>\u63a8\u8350\u65b9\u6cd5:<\/strong>
<\/span>\u9009\u7528Tris-EDTA\u7f13\u51b2\u6db2\uff08\u00a0PH9.0\uff09\u8fdb\u884c\u6297\u539f\u4fee\u590d\uff1b\u00a0\u4e00\u6297\u5728\u5ba4\u6e29\u4e0b\uff084\u2103) \u5b75\u80b2\u8fc7\u591c\u6216\uff0837\u2103\uff09\u6e7f\u76d2\u5b75\u80b22h\u3002
<\/span>\u514d\u75ab\u539f:<\/strong>
<\/span>\u4eba\u7ec6\u80de\u5468\u671f\u86cb\u767dD1\u86cb\u767dAla284\u5468\u56f4\u6b8b\u57fa\u7684\u5408\u6210\u80bd\u3002
<\/span>\u5b58\u50a8\u6eb6\u6db2:<\/strong>
<\/span>10mM HEPES\u94a0(pH 7.5)\u3001150 mM NaCl\u3001100\u00b5g\/ml BSA\u300150%\u7518\u6cb9\u548c\u5c11\u4e8e0.02%\u7684\u53e0\u6c2e\u5316\u94a0\u3002
<\/span>\u50a8\u5b58\u6761\u4ef6:<\/strong>
<\/span>-20\u00b0C\u00a0\u50a8\u5b58\u4e00\u5e74\u3002<\/span><\/p><\/figcaption><\/figure>\n\n\n\n
\u5b58\u50a8\u8bf4\u660e<\/strong>:<\/strong><\/p>\n\n\n\n
\u4ee5\u84dd\u51b0\u8fd0\u8f93\u3002\u6536\u8d27\u540e\uff0c\u8fdb\u884c\u5206\u88c5\uff0c\u5e76\u5728-25\u00b0C \u81f3-18\u00b0C \u6761\u4ef6\u4e0b\u50a8 \u5b58\u3002\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002<\/p>\n\n\n\n
\u80cc\u666f\u53c2\u8003\u6587\u732e<\/strong>:<\/strong><\/p>\n\n\n\n
\u00a01.Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.<\/p>\n\n\n\n
\u00a02.Sherr, C.J. (1996) Science 274, 1672-7.<\/p>\n\n\n\n
\u00a0<\/p>\n<\/div>\n\n\n\n
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\n\u6682\u65e0<\/p>\n\n\n\n
\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3>
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